■ Technical Information
■ Manuals
- C-Kit Ground Pro XRD: Protein crystallization kits for X-ray diffraction experiments.＞＞Click here for the C-Kit Ground Pro XRD instruction manual
- C-Kit Ground Pro ND: Protein crystallization kits for neutron diffraction experiments.＞＞Click here for the C-Kit Ground Pro ND instruction manual
■ Videos of experimental operation
■ 1.Preparing crystallization reagents
- A video for preparing crystallization reagents
■ 2.Loading the protein solution
- A video for loading the protein solution
■ 3.Sealing compound
- A video for a sealing compound
■ 4.Gel tube
- A video for a gel tube
■ C-Kit Education
- A video for C-Kit Education.
■ C-Tube LCDM
- A sample loading video for C-Tube LCDM.
■ Batch Method
- A sample loading video for the protein crystallization with batch method.
■ Publications
■ Space Experiment: Effects of microgravity
- Background of protein crystallization technologies in space. S. Takahashi, H. Tanaka. Int. J. Microgravity Sci. Appl. (2017). 34(1), 340103.(Japanese)
- Non-uniform diffractive quality of the crystals grown in microgravity and in terrestrial gravity. S. Takahashi, A. Nakagawa, N. Furubayashi, K. Inaka, K. Ohta, H. Tanaka. JJACG (2016). 43(2), 47–53.(Japanese)
- Numerical model of protein crystal growth in a diffusive field such as the microgravity environment. H. Tanaka, S. Sasaki, S. Takahashi, K. Inaka, Y. Wada, M. Yamada, K. Ohta, H. Miyoshi, T. Kobayashi and S. Kamigaichi, J. Synchrotron Rad. (2013). 20, 1003–1009.
- Numerical analysis of the diffusive field around a growing protein crystal in microgravity. K. Inaka, H. Tanaka, S. Takahashi, S. Sano, M. Sato, M. Shirakawa and Y. Yoshimura. Defect and Diffusion Forum Vols. 323-325 (2012). pp565-569.
- Controlling the Diffusive Field to Grow a Higher Quality Protein Crystal in Microgravity. H. Tanaka, K. Inaka, N. Furubayashi, M. Yamanaka, S. Takahashi, S. Sano, M. Sato, M. Shirakawa and Y. Yoshimura, Defect and Diffusion Forum Vols. 323-325 (2012). pp549-554.
- Prediction Method for Improvement of Protein Crystal Quality in Microgravity. H. Tanaka, K. Inaka, N. Furubayashi, M. Sato, S. Takahashi, M. Yamanaka, E. Hirota, S. Sano, T. Kobayashi and T. TanakaJ. Jpn. Soc. Microgravity Appl. (2008). 25(2),101-106 (Japanese).
- High-quality Crystal Growing under Space Environment. S. Takahashi and H. Tanaka, J. Cryst. Soc. Jpn. (2004). 46, 323-331 (Japanese).
- Numerical Analysis of the Depletion Zone Formation Around a Growing Protein Crystal. Hiroaki Tanaka, Koji Inaka, Shigeru Sugiyama, Sachiko Takahashi, Satoshi Sano, Masaru Sato, Susumu Yoshitomi. Transport Phenomena in Microgravity (2004), 1027(1), 10-19.
>■ Space Experiment: Container
- JCB-SGT Crystallization Devices Applicable to PCG Experiments and their Crystallization Conditions. Sachiko TAKAHASHI, Misako KOGA, Bin YAN, Naoki FURUBAYASHI, Masayuki KAMO, Koji INAKA and Hiroaki TANAKA. Int. J. Microgravity Sci. Appl. (2019). 36(1), 360107.
■ Space Experiment: Experimental project
- Essential Techniques for growing high-quality protein crystals for structural analysis in microgravity. K. Inaka, S. Takahashi, H. Tanaka. Int. J. Microgravity Sci. Appl. (2017). 34(1), 340104.(Japanese)
- JAXA protein crystallization in space: ongoing improvements for growing high-quality crystals. S. Takahashi, K. Ohta, N. Furubayashi, B. Yan, M. Koga, Y. Wada, M. Yamada, K. Inaka, H. Tanaka, H. Miyoshi, T. Kobayashi and S. Kamigaichi, J. Synchrotron Rad. (2013). 20, 968–97.
- Present State of High Quality Protein Crystal Growth in "Kibo" and Approach to Rational Crystallization in Space. S. Takahashi, B. Yan, N. Furubayashi, M. Masaki, K. Ohta, K. Inaka, H. Tanaka, T. Kobayashi and Y. Yoshimura. J. Jpn. Soc. Microgravity Appl. (2012).29(3),111-119.
- Spin-off of JAXA Protein Crystallization Experiment on International Space Station. H. Tanaka, S. Takahashi, N. Furubayashi, M. Yamanaka, Y. Bin, E. Hirota, S. Sano, M. Sato, K. Inaka, T. Kobayashi and T. Tanaka.J. Jpn. Soc. Microgravity Appl. (2010). 27(2), 104-112.
- JAXA-GCF project - High-quality protein crystals grown under microgravity environment for better understanding of protein structure. Sato, M., Tanaka, H., Inaka, K. et al. Microgravity Sci. Technol 18, 184–189 (2006).
■ Space Experiment: Results
- Bacterial Cellulose Production in Space. Akiko Tanaka, Tomoya Imai, Misako Koga, Naoki Sunagawa, Kiyohiko Igarashi, Kenji Tajima, Hiroaki Tanaka. Int. J. Microgravity Sci. Appl. 41(3) (2024) 410302.
- Effect of Impurity Concentration on the Precipitation of Atorvastatin Calcium Under Microgravity on the International Space Station. Makoto Otsuka, Yota Kuroyanagi, Tetsuo Sasaki, Masakazu Matsumoto, Yutaro Hirose, Hiroaki Tanaka and Naohiro Sato. Colloids and Surfaces A: Physicochemical and Engineering Aspects (2024) 134553.
- Microgravity environment grown crystal structure information based engineering of direct electron transfer type glucose dehydrogenase. Junko Okuda-Shimazaki, Hiromi Yoshida, Inyoung Lee, Katsuhiro Kojima, Nanoha Suzuki, Wakako Tsugawa, Mitsugu Yamada, Koji Inaka, Hiroaki Tanaka and Koji Sode. Communications Biology 5 (2022) 1334.
- Fragment-based discovery of the first nonpeptidyl inhibitor of an S46 family peptidase. Yasumitsu Sakamoto, Yoshiyuki Suzuki, Akihiro Nakamura, Yurie Watanabe, Mizuki Sekiya, Saori Roppongi, Chisato Kushibiki, Ippei Iizuka, Osamu Tani, Hitoshi Sakashita, Koji Inaka, Hiroaki Tanaka, Mitsugu Yamada, Kazunori Ohta, Nobuyuki Honma, Yosuke Shida, Wataru Ogasawara, Mayumi Nakanishi-Matsui, Takamasa Nonaka, Hiroaki Gouda & Nobutada Tanaka. Scientific Reports volume 9, Article number: 13587 (2019).
- Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery. Itoh T, Hibi T, Suzuki F, Sugimoto I, Fujiwara A, et al. PLOS ONE (2016) 11(12): e0167310.
- “Newton’s cradle” proton relay with amide–imidic acid tautomerization in inverting cellulase visualized by neutron crystallography. Akihiko Nakamura, Takuya Ishida, Katsuhiro Kusaka, Taro Yamada, Shinya Fushinobu, Ichiro Tanaka, Satoshi Kaneko, Kazunori Ohta, Hiroaki Tanaka, Koji Inaka, Yoshiki Higuchi, Nobuo Niimura, Masahiro Samejima and Kiyohiko Igarashi. SCIENCE ADVANCES21 AUG 2015 : E1500263.
- Structural and mutational analyses of dipeptidyl peptidase 11 from Porphyromonas gingivalis reveal the molecular basis for strict substrate specificity. Yasumitsu Sakamoto, Yoshiyuki Suzuki, Ippei Iizuka, Chika Tateoka, Saori Roppongi, Mayu Fujimoto, Koji Inaka, Hiroaki Tanaka, Mitsugu Yamada, Kazunori Ohta, Hiroaki Gouda, Takamasa Nonaka, Wataru Ogasawara & Nobutada Tanaka. Scientific Reports volume 5, Article number: 11151 (2015).
- S46 Peptidases are the First Exopeptidases to be Members of Clan PA. Yasumitsu Sakamoto, Yoshiyuki Suzuki, Ippei Iizuka, Chika Tateoka, Saori Roppongi, Mayu Fujimoto, Koji Inaka, Hiroaki Tanaka, Mika Masaki, Kazunori Ohta, Hirofumi Okada, Takamasa Nonaka, Yasushi Morikawa, Kazuo T. Nakamura, Wataru Ogasawara & Nobutada Tanaka . Sci Rep 4, 4977 (2014).
- High-Quality Protein Crystal Growth of Mouse Lipocalin- Type Prostaglandin D Synthase in Microgravity. K. Inaka, S. Takahashi, K. Aritake, T. Tsurumura, N. Furubayashi, B. Yan, E. Hirota, S. Sano, M. Sato, T. Kobayashi, Y. Yoshimura, H. Tanaka and Y. Urade, Cryst Growth Des. (2011). June 1; 11(6): 2107-2111.
- Improvement in the quality of hematopoietic prostaglandin D synthase crystals in a microgravity environment. H. Tanaka, T. Tsurumura, K. Aritake, N. Furubayashi, S. Takahashi, M. Yamanaka, E. Hirota, S. Sano, M. Sato, T. Kobayashi, T. Tanaka, K. Inaka and Y. Urade. J. Synchrotron Rad. (2011). 18, 88-91.
- High-quality crystals of human haematopoietic prostaglandin D synthase with novel inhibitors. S. Takahashi, T. Tsurumura, K. Aritake, N. Furubayashi, M. Sato, M. Yamanaka, E. Hirota, S. Sano, T. Kobayashi, T. Tanaka, K. Inaka, H. Tanaka and Y. Urade. Acta Cryst. (2010). F66, 846-850.
- Useful Results of JAXA Protein Crystal Growth Experiment in ISS. S. Takahashi, S. Sano, M. Sato, E. Hirota, N. Furubayashi, Y. Bin, T. Kobayashi, I. Koji, H. Tanaka and T. TanakaJ. Jpn. Soc. Microgravity Appl. (2010). 27(2), 98-103.
- High-quality Protein Crystal for Precise Structual Analysis Obtained by JAXA Crystallization Experiments in Space. S. Takahashi, N. Furubayashi, T. Tsurumura, K. Aritake, M. Sato, M. Yamanaka, E. Hirota, S. Sano, K. Inaka, H. Tanaka, T. Kobayashi, Y. Urade and T. Tanaka.J. Jpn. Soc. Microgravity Appl. (2008).25(2),141-146.
- Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions. H. Tanaka, T. Umehara, K. Inaka, S. Takahashi, R. Shibata, Y. Bessho, M. Sato, S. Sugiyama, E. Fusatomi, T. Terada, M. Shirouzu, S. Sano, M. Motohara, T. Kobayashi, T. Tanaka, A. Tanaka and S. Yokoyama. Acta Cryst. (2007). F63, 69-73.
■ Crystallization-related technologies
- Through Diffusion Measurements of Molecules to a Numerical Model for Protein Crystallization in Viscous Polyethylene Glycol Solution. Hiroaki Tanaka, Rei Utata, Keiko Tsuganezawa, Sachiko Takahashi and Akiko Tanaka. Crystals (2022). 12, 881.
- Novel Device and Strategy for Growing Large, High-Quality Protein Crystals by Controlling Crystallization Conditions. Naoki Tanigawa, Sachiko Takahashi, Bin Yan, Masayuki Kamo, Naoki Furubayashi, Koji Kubota, Koji Inaka and Hiroaki Tanaka. Crystals (2021). 11, 1311.
- Semi-empirical model to estimate ideal conditions for the growth of large protein crystals. Hirohiko Nakamura, Sachiko Takahashi, Koji Inaka and Hiroaki Tanaka. Acta Cryst. D. 76 (2020) 1174-1183.
- Methods for Obtaining Better Diffractive Protein Crystals: From Sample Evaluation to Space Crystallization. Yoshinobu. Hashizume, Koji Inaka, Naoki Furubayashi, Masayuki Kamo, Sachiko Takahashi and Hiroaki Tanaka. Crystals (2020). 10, 78.
- A novel handling-free method of mounting single protein crystals for synchrotron structure analyses at room temperature. Yoshihisa Suzuki, Kumi Torii, Koji Inaka, Takahisa Fujiwara, Hiroaki Tanaka and Yoshitomo Arai. Review of Scientific Instruments 90, 054101 (2019).
- Optimization of salt concentration in PEG-based crystallization solutions. M. Yamanaka, K. Inaka, N. Furubayashi, M. Matsushima, S. Takahashi, H. Tanaka, S. Sano, M. Sato, T. Kobayashi and T. Tanaka. J. Synchrotron Rad. (2011). 18, 84-87.
- Diffusion coefficient of the protein in various crystallization solutions: The key to growing high-quality crystals in space. Hiroaki Tanaka, Sachiko Takahashi, Mari Yamanaka, Izumi Yoshizaki, Masaru Sato, Satoshi Sano, Moritoshi Motohara, Tomoyuki Kobayashi, Susumu Yoshitomi, Tetsuo Tanaka and Seijiro Fukuyama. Microgravity Sci. Technol 18, 91–94 (2006).
- A simplified counter diffusion method combined with a 1D simulation program for optimizing crystallization conditions. H. Tanaka, K. Inaka, S. Sugiyama, S. Takahashi, S. Sano, M. Sato and S. Yoshitomi. J. Synchrotron Rad. (2004). 11, 45-48.